Composite
Part:BBa_K4388008
Designed by: Tomas Lopes Group: iGEM22_KCL_UK (2022-10-09)
Transcriptional Unit 4 in Synthetic Operon for Pterostilbene Production
This composite part is the fourth transcriptional unit (TU) of the 4-gene synthetic operon constructed for pterostilbene production from L-tyrosine as precursor. This part includes a linker (replacing promoter) and an RBS (BBa_K4388011), a Vitis vinifera resveratrol O-methyltransferase mutant (BBa_K4388004), and a strong double forward transcriptional terminator (BBa_B0015). This composite part was assembled via JUMP type IIS assembly whereby the three above-mentioned basic parts were ligated into the pJUMP29-1D vector.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 170
Illegal BamHI site found at 420 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |